custom built zeiss axio observer 3 microscope stage  (Carl Zeiss)

Journal: Nature Neuroscience

Article Title: Rapid temporal processing in the olfactory bulb underlies concentration-invariant odor identification and signal decorrelation

doi: 10.1038/s41593-026-02250-y

Figure Lengend Snippet: A . 2P microscope equipped with a DMD-based pattern stimulation setup and wide field cameras for alignment calibration. Camera 1 was positioned in the conjugate plane of the DMD and was used for alignment of the DMD projected patterns to the 2 P imaging. Camera 2 and Objective 2 are temporarily placed below the sample plane and used to characterize the axial spread of projected patterns. DM - dichoric mirror. B . Left : Images of illuminated areas of different size square patches (NxN) of active DMD pixels taken by Camera 1. Right: Estimated and measured full-width half-maximum (FWHM) of illuminated areas for different patch sizes. C . Left: Images of projected patterns of 20×20 DMD pixels at different positions of the Objective 1 taken by Camera 2. Right: Intensity profiles of projected patterns along the x-axis. Di . 2 P images of the glomerular layer with manually drawn glomerular ROIs. Dii . Corresponding DMD mask patterns; Diii . Image of the glomerular mask pattern with Camera 1. Div . Images of the glomerular mask pattern with Camera 2 at different position of the Objective 1.

Article Snippet: This algorithm attempted to minimize the difference between the reference image and the FOV by iteratively moving the microscope stage (Thorlabs, cat. no. PLS-XY) to reduce the residual displacement computed using a rigid motion correction package (NoRMCorre, Flatiron Institute) .

Techniques: Microscopy, Imaging